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Histone 3 antibody (H3K9ac)

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IHC, IF, ChIP, IP and ChIP-seq. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN4903918

Quick Overview for Histone 3 antibody (H3K9ac) (ABIN4903918)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)
  • Binding Specificity

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    H3K9ac

    Characteristics

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

    Purification

    Affinity purification

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200, CHIP 1:20-1:100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze/thaw cycles.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C.
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Gene ID

    8290

    UniProt

    Q16695
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