PODXL antibody

Catalog No. ABIN4913137

This Mouse Monoclonal antibody specifically detects PODXL in WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PODXL antibody (ABIN4913137)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2C4

Product Details anti-PODXL Antibody

Cross-Reactivity: Human

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL

Background:

Synonyms: PC, PCLP, Gp200, PCLP-1, Podocalyxin, GCTM-2 antigen, Podocalyxin-like protein 1, PODXL, PCLP1

Background: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Gene ID: 5420

UniProt: O00592

Pathways: Tube Formation