ORAI1 antibody (C-Term)

Catalog No. ABIN492545

The Mouse Monoclonal anti-ORAI1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect ORAI1 in samples from Human, Mouse and Rat.

Quick Overview for ORAI1 antibody (C-Term) (ABIN492545)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 3F6H5

Product Details anti-ORAI1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes ORAI1. This antibody is predicted to have no cross-reactivity with ORAI2 or ORAI3.

Characteristics: Synonyms: ORAI-1, CRACM1, TMEM142A, Protein orai-1, Transmembrane protein 142A, Calciumrelease-activated calcium channel protein 1

Purification: Immunoaffinity Chromatography.

Immunogen: A 16 amino acid peptide from near the carboxy terminus of Human ORAI1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ORAI1 antibody can be used for detection of ORAI1 at 1-2 μg/mL. Positive Control: Human Ovary Tissue Lysate. Immunohistochemistry: 2.5 μg/mL. Positive Control: Human Spleen Tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1

Background: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels (1 for review). ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry (2). It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor (3). ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE.Synonyms: CRACM1, Calcium release-activated calcium channel protein 1, ORAI-1, Protein orai-1, TMEM142A, Transmembrane protein 142A

Gene ID: 84876

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling