Streptavidin antibody

Catalog No. ABIN492627

This anti-Streptavidin antibody is a Mouse Monoclonal antibody detecting Streptavidin in WB, EIA and IP. Suitable for Streptomyces avidinii.

Quick Overview for Streptavidin antibody (ABIN492627)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Mouse

Clonality: Monoclonal

Conjugate: This Streptavidin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Clone: 36G3

Product Details anti-Streptavidin Antibody

Characteristics: Synonyms: StAv

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Immunogen: Streptavidin

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 0.05 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 0.05-1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for Streptavidin

Target: Streptavidin

Background: Streptavidin is a tetrameric protein that binds tightly to a small growth factor Biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the Vitamin Biotin, the dissociation constant (Kd) of the Biotin-Streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of Biotin and biotinylated molecules has made Streptavidin one of the most important components in diagnostics and laboratory kits.Synonyms: StAv

UniProt: P22629