GJB1 antibody (Cytoplasmic Loop)

Catalog No. ABIN4949578

The Mouse Monoclonal anti-GJB1 antibody has been validated for WB, IF, FACS and IHC (fro). It is suitable to detect GJB1 in samples from Human, Rat and Mouse.

Quick Overview for GJB1 antibody (Cytoplasmic Loop) (ABIN4949578)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M12-13

Product Details anti-GJB1 Antibody

Binding Specificity: Cytoplasmic Loop

Purification: Protein G affinity chromatography

Immunogen: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: Connexin 32 / GJB1

Background: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Pathways: Cell-Cell Junction Organization