Desmocollin 2+3 (Extracellular Domain) antibody

Catalog No. ABIN4949601

This Mouse Monoclonal antibody specifically detects in IF, WB, FACS and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Desmocollin 2+3 (Extracellular Domain) antibody (ABIN4949601)

Target: Desmocollin 2+3

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7G6

Product Details

Binding Specificity: Extracellular Domain

Purification: Protein G affinity chromatography

Immunogen: A recombinant protein corresponding to the extracellular domain of human Desmocollin 2 was used as the immunogen for the Desmocollin 2/3 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Desmocollin 2/3 antibody should be determined by the researcher.\. WB: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Desmocollin 2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Desmocollin 2+3

Alternative Name: Desmocollin 2/3

Background: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of Desmocollin-2 varies in the basal and suprabasal layers. And Desmocollin-3 is expressed more evenly throughout the suprabasal layers.