Thymidine Phosphorylase antibody

Catalog No. ABIN4949902

The Mouse Monoclonal anti-Thymidine Phosphorylase antibody is suitable to detect Thymidine Phosphorylase in samples from Human, Rat and Mouse. It has been validated for WB, IP and IHC (p).

Quick Overview for Thymidine Phosphorylase antibody (ABIN4949902)

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thymidine Phosphorylase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM322

Product Details anti-Thymidine Phosphorylase Antibody

Purification: Protein G affinity chromatography

Immunogen: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.\. IP: 1-2 μg/500 μg lysate,IHC (FFPE): 1-2 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Thymidine Phosphorylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: Thymidine Phosphorylase / PD-ECGF

Background: Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2