TIMP2 antibody (N-Term)

Catalog No. ABIN4949918

This anti-TIMP2 antibody is a Mouse Monoclonal antibody detecting TIMP2 in WB, IF and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for TIMP2 antibody (N-Term) (ABIN4949918)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM356

Product Details anti-TIMP2 Antibody

Binding Specificity: N-Term

Purification: Protein G affinity chromatography

Immunogen: An amino acid sequence from the N-terminal region of the human protein was used as the immunogen for this TIMP2 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.\. IHC (FFPE): 0.5-1 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL,IF: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the TIMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background: It recognizes a protein of 21  kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2). It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72  kDa Type IV collagenase (also known as MMP2 or gelatinase A). It also has affinity for the active form of 92  kDa Type IV collagenase (also known as MMP9 or gelatinase B). TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Pathways: cAMP Metabolic Process