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Recombinant Histone 3 antibody (acLys18)

Name: Recombinant Histone 3 antibody (acLys18)
SKU: ABIN4953325
Price: 728.75 USD
Availability: InStock

The Rabbit Monoclonal anti-Histone 3 antibody has been validated for WB, IHC, ELISA, ChIP and ICC. It is suitable to detect Histone 3 in samples from All Species.

Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K18ac antibody at 0. (Recombinant Histone 3 antibody (acLys18))

ChIP performed on HeLa cells with or without sodium butyrate treatment using recombinant H3K18ac antibody (5ug). (Recombinant Histone 3 antibody (acLys18))

ICC staining of human HepG2 cells using recombinant H3K18ac antibody. (Recombinant Histone 3 antibody (acLys18))

ICC

The recombinant H3K18ac antibody specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). (Recombinant Histone 3 antibody (acLys18))

ICC/IF test of HeLa cells treated with sodium butyrate using recombinant H3K18ac antibody (red). (Recombinant Histone 3 antibody (acLys18))

ICC

5 images

Catalog No. ABIN4953325

Quick Overview for Recombinant Histone 3 antibody (acLys18) (ABIN4953325)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Antibody Type

Recombinant Antibody

Reactivity

1432
940
841
39
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32
25
23
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10
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All Species

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1080
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339
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191
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145
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115
48
48
37
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26
25
15
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7
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4
3
1
1
1
1

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Clone

RM166

Discover our top product H3 Primary Antibody

Binding Specificity
51

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acLys18

Characteristics

This recombinant H3K18ac antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.

Purification

Protein A purified from animal origin-free supernatant

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys18) was used as the immunogen for this recombinant H3K18ac antibody.

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K18ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,ChIP: 2 μg/mL-10 μg/mg of lysate,Immunohistochemistry: 1-10 μg/mL (1),ELISA: 0.2-1 μg/mL

Restrictions

For Research Use only
Buffer

1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store the recombinant H3K18ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

This recombinant H3K18ac antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.

Gene ID

8350

UniProt

P84243