Histone Cluster 3, H3 (HIST3H3) (meLys79) antibody

Details for Product No. ABIN4953342
Target
Binding Specificity
meLys79
8
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
All Species
43
9
1
1
Host
Rabbit
52
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)
52
52
31
21
18
5
1
1
Options
Immunogen A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me1 antibody.
Clone RM147
Isotype IgG
Characteristics This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.
Purification Protein A purified from animal origin-free supernatant
Target
Alternative Name Histone H3 (HIST3H3 Antibody Abstract)
Background This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.
Gene ID 8350
UniProt P84243
Application Notes The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.2-1 μg/mL,ChIP: 2-10 μg/mg of lysate,ELISA: 0.2-1 μg/mL
Restrictions For Research Use only
Buffer 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the recombinant H3K79me1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Images
Image no. 1 for anti-Histone Cluster 3, H3 (HIST3H3) (meLys79) antibody (ABIN4953342) Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) usin...
Image no. 2 for anti-Histone Cluster 3, H3 (HIST3H3) (meLys79) antibody (ABIN4953342) The recombinant H3K79me1 antibody specifically reacts to Histone H3 monomethylated at...