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Histone 3 antibody (H3) H3K27me Primary Antibody

H3 Reactivity: Human, Mouse, Rat ChIP, IF, IP, IHC, WB Host: Rabbit Polyclonal unconjugated
Pubmed (14 references)
Catalog No. ABIN3023269
$735.08
Plus shipping costs $45.00
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  • Target See all Histone 3 (H3) Antibodies
    Histone 3 (H3)
    Binding Specificity
    • 70
    • 50
    • 47
    • 47
    • 46
    • 41
    • 39
    • 39
    • 38
    • 37
    • 35
    • 34
    • 34
    • 32
    • 30
    • 30
    • 28
    • 27
    • 26
    • 24
    • 21
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    • 18
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    • 16
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    • 15
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    • 14
    • 13
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    • 12
    • 12
    • 12
    • 12
    • 12
    • 12
    • 11
    • 10
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
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    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    H3K27me
    Reactivity
    • 1567
    • 1080
    • 980
    • 59
    • 50
    • 44
    • 44
    • 44
    • 42
    • 40
    • 39
    • 35
    • 32
    • 23
    • 22
    • 11
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 1330
    • 304
    • 9
    • 5
    Rabbit
    Clonality
    • 1256
    • 391
    • 1
    Polyclonal
    Conjugate
    • 976
    • 86
    • 65
    • 62
    • 62
    • 62
    • 62
    • 62
    • 37
    • 35
    • 21
    • 18
    • 18
    • 17
    • 17
    • 17
    • 12
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Histone 3 antibody is un-conjugated
    Application
    • 1157
    • 613
    • 507
    • 446
    • 271
    • 240
    • 230
    • 218
    • 188
    • 161
    • 145
    • 141
    • 116
    • 48
    • 38
    • 38
    • 32
    • 19
    • 14
    • 11
    • 7
    • 6
    • 6
    • 1
    • 1
    Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
    Purification
    Affinity purification
    Immunogen
    A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
    Isotype
    IgG
  • Application Notes
    Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:50-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze / thaw cycles
    Storage
    -20 °C/-80 °C
  • Wang, Wang, Lin, Ye, Li, Tu, Shen, Li, Yang, Zhang: "Evolutionary dynamics of 3D genome architecture following polyploidization in cotton." in: Nature plants, Vol. 4, Issue 2, pp. 90-97, (2018) (PubMed).

    Cheng, Tan, Lu, Liu, Li, Yuan, Zhao, Zhou: "WOX11 recruits a histone H3K27me3 demethylase to promote gene expression during shoot development in rice." in: Nucleic acids research, Vol. 46, Issue 5, pp. 2356-2369, (2018) (PubMed).

    Wu, Tian, Zhang, Tong, Huang, Li, Zhao, Tang, Yuan, Wang, Fang, Gao, Hu, Li, Qin, Yao, Chen, Chen, Zhang, Liu, Sun, Chen, Wong, Ge, Chen, Ji: "In vivo CRISPR screening unveils histone demethylase UTX as an important epigenetic regulator in lung tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17, pp. E3978-E3986, (2018) (PubMed).

    Dumasia, Kumar, Deshpande, Balasinor: "Estrogen, through estrogen receptor 1, regulates histone modifications and chromatin remodeling during spermatogenesis in adult rats." in: Epigenetics, Vol. 12, Issue 11, pp. 953-963, (2018) (PubMed).

    Yu, Liu, Liu, Wang, Liu, Miao, Du, Yang: "Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 6132, (2018) (PubMed).

    Song, Li, Wang, Du, Yang: "DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes." in: Scientific reports, Vol. 7, Issue 1, pp. 1687, (2018) (PubMed).

    Tang, Guo, Zhu, Huang, Liu, Cai, Yu, Wang: "Metformin inhibits ovarian cancer via decreasing H3K27 trimethylation." in: International journal of oncology, Vol. 52, Issue 6, pp. 1899-1911, (2018) (PubMed).

    Cao, Liu, Yue, Liu, Pei, Gu, Wang, Jia: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, (2018) (PubMed).

    Wang, Tu, Lin, Lin, Wang, Yang, Ye, Shen, Li, Zhang, Zhou, Nie, Li, Guo, Ma, Huang, Jin, Zhu, Yang, Min, Yuan, Zhang, Lindsey, Zhang: "Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication." in: Nature genetics, Vol. 49, Issue 4, pp. 579-587, (2017) (PubMed).

    Zhou, Liu, Zhou, Zhou, Zhao, Li, Zhou: "Cooperation between the H3K27me3 Chromatin Mark and Non-CG Methylation in Epigenetic Regulation." in: Plant physiology, Vol. 172, Issue 2, pp. 1131-1141, (2017) (PubMed).

    Kumar, Dumasia, Deshpande, Balasinor: "Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators." in: The Journal of steroid biochemistry and molecular biology, Vol. 171, pp. 66-74, (2017) (PubMed).

    Su, Li, Lei, Wang, Zhao, Cai, Wang, Li, Wu: "The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT." in: Journal of cell science, Vol. 129, Issue 12, pp. 2343-53, (2017) (PubMed).

    Yi, Guo, Guo, Sun, Yang, Wang, Li, Xie, Cai, Wang: "EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion." in: Scientific reports, Vol. 7, Issue 1, pp. 3568, (2017) (PubMed).

    Liu, Zhou, Wang, Ye, Zhao, Xu, Zhou, Tan, Cheng, Zhou: "Regulation of histone methylation and reprogramming of gene expression in the rice inflorescence meristem." in: The Plant cell, Vol. 27, Issue 5, pp. 1428-44, (2016) (PubMed).

  • Target
    Histone 3 (H3)
    Alternative Name
    Histone H3 (H3 Products)
    Synonyms
    H-3, histocompatibility 3, H3
    Background
    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
    Synonyms: HIST1H3J , H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3
    Gene ID
    8290
    UniProt
    Q16695
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