Recombinant Histone H4 antibody (meArg3)

Catalog No. ABIN4953355

The Rabbit Monoclonal anti-Histone H4 antibody has been validated for WB, ELISA and ICC. It is suitable to detect Histone H4 in samples from All Species.

Quick Overview for Recombinant Histone H4 antibody (meArg3) (ABIN4953355)

Target: Histone H4

Antibody Type: Recombinant Antibody

Reactivity: All Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: RM195

Product Details anti-Histone H4 Antibody

Binding Specificity: meArg3

Characteristics: This recombinant H4R3me1 antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A monomethyl-peptide corresponding to Monomethyl-Histone H4 (Arg3) was used as the immunogen for this recombinant H4R3me1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant H4R3me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant H4R3me1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for Histone H4

Target: Histone H4

Background: This recombinant H4R3me1 antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.

Gene ID: 121504

UniProt: P62805