For best experience we recommend to activate Javascript in your browser.

Recombinant Histone 3 antibody (Lys4, N-Term)

Name: Recombinant Histone 3 antibody (Lys4, N-Term)
SKU: ABIN4953365
Price: 728.75 USD
Availability: InStock

This anti-Histone 3 antibody is a Rabbit Monoclonal antibody detecting Histone 3 in WB, ELISA and ICC. Suitable for All Species.

Western blot test of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated and untreated with Nocodazole, and recombinant Histone H3. (Recombinant Histone 3 antibody (Lys4, N-Term))

This recombinant Histone H3 antibody specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. (Recombinant Histone 3 antibody (Lys4, N-Term))

2 images

Catalog No. ABIN4953365

Quick Overview for Recombinant Histone 3 antibody (Lys4, N-Term) (ABIN4953365)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Antibody Type

Recombinant Antibody

Reactivity

1345
909
811
40
39
39
34
33
26
23
21
21
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

All Species

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1028
418
373
323
253
202
188
187
163
159
147
127
115
58
48
48
35
22
16
14
7
7
6
4
3
1
1
1
1

Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone

RM186

Discover our top product H3 Primary Antibody

Binding Specificity
52

50

48

44

39

37

37

36

35

35

34

33

32

31

31

30

23

23

23

22

21

19

18

17

17

17

16

16

16

16

15

15

14

13

13

13

13

12

12

12

11

11

10

10

9

9

9

8

8

8
Lys4, N-Term

Characteristics

This recombinant Histone H3 antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The recombinant Histone H3 antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

Purification

Protein A purified from animal origin-free supernatant

Immunogen

An unmodified peptide corresponding to the N-terminus of Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IF, IHC, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 1-2 μg/mL,ELISA: 0.5-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL

Restrictions

For Research Use only
Buffer

1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store the recombinant Histone H3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

This recombinant Histone H3 antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The recombinant Histone H3 antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

Gene ID

8350

UniProt

Q16695