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Alkaline Phosphatase antibody (AA 274-304)

The Rabbit Polyclonal anti-Alkaline Phosphatase antibody has been validated for WB, IHC, IF and ELISA. It is suitable to detect Alkaline Phosphatase in samples from Human.
Catalog No. ABIN3029903

Quick Overview for Alkaline Phosphatase antibody (AA 274-304) (ABIN3029903)

Target

See all Alkaline Phosphatase (ALP) Antibodies
Alkaline Phosphatase (ALP)

Reactivity

  • 74
  • 19
  • 13
  • 12
  • 10
  • 1
  • 1
Human

Host

  • 64
  • 29
  • 2
  • 1
  • 1
Rabbit

Clonality

  • 49
  • 48
Polyclonal

Conjugate

  • 47
  • 13
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This Alkaline Phosphatase antibody is un-conjugated

Application

  • 56
  • 35
  • 20
  • 15
  • 12
  • 11
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  • 7
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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
  • Binding Specificity

    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 274-304

    Purification

    Antigen affinity purified

    Immunogen

    A portion of amino acids 274-304 from human ALPI was used as the immunogen for this anti-Alkaline Phosphatase antibody. This sequence is 87% homologous in human ALPP.

    Isotype

    Ig Fraction
  • Application Notes

    Titration of the anti-Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-100,Immunofluorescence: 1:100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    In 1X PBS, pH 7.4, with 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Aliquot the anti-Alkaline Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
  • Target

    Alkaline Phosphatase (ALP)

    Alternative Name

    Alkaline Phosphatase

    Background

    There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

    UniProt

    P09923
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