CAD antibody (AA 780-809)

Catalog No. ABIN3030276

This anti-CAD antibody is a Rabbit Polyclonal antibody detecting CAD in IHC, WB, ELISA and FACS. Suitable for Human.

Quick Overview for CAD antibody (AA 780-809) (ABIN3030276)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAD antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CAD Antibody

Binding Specificity: AA 780-809

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 780-809 from the human protein was used as the immunogen for this CAD antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CAD antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CAD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Alternative Name: CAD

Background: The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

UniProt: P27708

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process