CD8 alpha antibody (AA 150-180)

Catalog No. ABIN3030431

The Rabbit Polyclonal anti-CD8 alpha antibody has been validated for FACS, IHC, WB and ELISA. It is suitable to detect CD8 alpha in samples from Human.

Quick Overview for CD8 alpha antibody (AA 150-180) (ABIN3030431)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 150-180

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 150-180 from the human protein was used as the immunogen for this CD8 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CD8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

UniProt: P01732

Pathways: TCR Signaling