GST-Tag antibody

Catalog No. ABIN3029803

This Mouse Monoclonal antibody specifically detects GST-Tag in WB and ELISA. It exhibits reactivity toward Schistosoma japonicum.

Quick Overview for GST-Tag antibody (ABIN3029803)

Target: GST-Tag

Reactivity: Schistosoma japonicum

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 9AT106

Product Details anti-GST-Tag Antibody

Purification: Purified

Immunogen: Purified recombinant fusion protein was used to produced this monoclonal GST tag antibody.

Isotype: IgG1

Application Details

Application Notes: Titration of the GST Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GST Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GST-Tag

Target: GST-Tag

Alternative Name: GST Tag

Target Type: Tag

Background: Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.