ILK antibody (AA 391-421)

Catalog No. ABIN3031391

The Rabbit Polyclonal anti-ILK antibody has been validated for WB and ELISA. It is suitable to detect ILK in samples from Human and Mouse.

Quick Overview for ILK antibody (AA 391-421) (ABIN3031391)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ILK Antibody

Binding Specificity: AA 391-421

Cross-Reactivity (Details): Expected species reactivity: Rat, Bovine

Purification: Purified

Immunogen: A portion of amino acids 391-421 from the human protein was used as the immunogen for this ILK2/ILK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the ILK2/ILK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ILK2/ILK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK2/ILK1

Background: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) is an ankyrin repeat containing 51 kDa receptor-proximate serine-threonine kinase (1), with a reported migration rate of 59K. This 451 amino acid protein interacts with the cytoplasmic domain of the beta-1 integrin subunit and contains sequence motifs found in pleckstrin homology domains capable of interacting with phosphoinositide lipids. ILK is an upstream regulator of Pi(3)K dependant activation of protein kinase B (PKB/AKT) and inhibition of glycogen synthase kinase 3 (GSK-3). ILK2 expression is associated with mediation of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. ILK2 is overexpressed in some highly invasive tumor cell lines.

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration