LATS1 antibody (AA 1-30)

Catalog No. ABIN3031593

This anti-LATS1 antibody is a Rabbit Polyclonal antibody detecting LATS1 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for LATS1 antibody (AA 1-30) (ABIN3031593)

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LATS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-LATS1 Antibody

Binding Specificity: AA 1-30

Purification: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this LATS1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the LATS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the LATS1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LATS1

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Alternative Name: LATS1

Background: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

UniProt: O95835

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location