NPC1 antibody (AA 591-620)

Catalog No. ABIN3031998

This anti-NPC1 antibody is a Rabbit Polyclonal antibody detecting NPC1 in ELISA, WB, IHC and IF. Suitable for Human.

Quick Overview for NPC1 antibody (AA 591-620) (ABIN3031998)

Target: NPC1 (Niemann-Pick Disease, Type C1 (NPC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPC1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NPC1 Antibody

Binding Specificity: AA 591-620

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 591-620 from the human protein was used as the immunogen for this NPC1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the NPC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NPC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for NPC1

Target: NPC1 (Niemann-Pick Disease, Type C1 (NPC1))

Alternative Name: NPC1

Background: This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.

UniProt: O15118