PFKFB3 antibody (AA 454-484)

Catalog No. ABIN3029823

This anti-PFKFB3 antibody is a Rabbit Polyclonal antibody detecting PFKFB3 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for PFKFB3 antibody (AA 454-484) (ABIN3029823)

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PFKFB3 Antibody

Binding Specificity: AA 454-484

Cross-Reactivity (Details): Expected species reactivity: Rat

Purification: Purified

Immunogen: A portion of amino acids 454-484 from the human protein was used as the immunogen for this PFKFB3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PFKFB3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PFKFB3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PFKFB3

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Alternative Name: PFKFB3

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

UniProt: Q16875

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process