PGK1 antibody (AA 117-145)

Catalog No. ABIN3032224

This Rabbit Polyclonal antibody specifically detects PGK1 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for PGK1 antibody (AA 117-145) (ABIN3032224)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PGK1 Antibody

Binding Specificity: AA 117-145

Cross-Reactivity (Details): Expected species reactivity: Primate, Rat

Purification: Purified

Immunogen: A portion of amino acids 117-145 from the human protein was used as the immunogen for this PGK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1

Background: Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process