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Histone 3 antibody (pSer10)

Name: Histone 3 antibody (pSer10)
SKU: ABIN3031216
Price: 649.42 USD
Availability: InStock

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, ELISA and DB. It exhibits reactivity toward Human.

Western blot analysis of extracts from HeLa cells, untreated or treated with FBS and Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right). (Histone 3 antibody (pSer10))

Dot blot analysis of phospho-Histone H3 antibody. (Histone 3 antibody (pSer10))

Western blot analysis of lysate from HeLa cell line using phospho-Histone H3 antibody at 1:1000. (Histone 3 antibody (pSer10))

Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody (Histone 3 antibody (pSer10))

Western blot analysis of lysate from HeLa cell line, untreated or treated with FBS + Calyculin A (100ng/ml), using phospho-Histone H3 antibody (Histone 3 antibody (pSer10))

Western blot analysis of extracts from HeLa cells, untreated or treated with FBS + Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right). (Histone 3 antibody (pSer10))

9 images

Catalog No. ABIN3031216

Quick Overview for Histone 3 antibody (pSer10) (ABIN3031216)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

1344
909
811
41
39
39
34
33
26
23
21
21
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1028
418
373
323
253
201
188
187
163
159
148
127
115
58
48
48
35
22
16
14
7
7
6
4
3
1
1
1
1

Western Blotting (WB), ELISA, Dot Blot (DB)

Discover our top product H3 Primary Antibody

Binding Specificity
51

50

48

44

39

37

37

36

35

35

34

33

32

31

31

30

23

23

23

22

22

19

18

17

17

17

16

16

16

16

15

15

14

13

13

13

13

12

12

12

11

11

10

10

9

9

9

8

8

8
pSer10

Purification

Antigen affinity purified

Immunogen

This phospho-Histone H3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS10 of human HIST1H3A.

Isotype

Ig Fraction
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IF, IHC, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

Titration of the phospho-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-4000,Dot blot: 1:500

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

UniProt

P68431