Recoverin antibody (AA 169-200)

Catalog No. ABIN3032452

This Rabbit Polyclonal antibody specifically detects Recoverin in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Recoverin antibody (AA 169-200) (ABIN3032452)

Target: Recoverin (RCVRN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Recoverin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Recoverin Antibody

Binding Specificity: AA 169-200

Purification: Purified

Immunogen: A portion of amino acids 169-200 from the human protein was used as the immunogen for this Recoverin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Recoverin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Recoverin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Recoverin

Target: Recoverin (RCVRN)

Alternative Name: Recoverin

Background: Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.

UniProt: P35243

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction