SUMO2 antibody

Catalog No. ABIN3032647

This Mouse Monoclonal antibody specifically detects SUMO2 in WB, ELISA and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for SUMO2 antibody (ABIN3032647)

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUMO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 973CT8-1-1

Product Details anti-SUMO2 Antibody

Cross-Reactivity (Details): Expected species reactivity: Bovine, Chicken, Hamster, Mouse, Pig, Primate

Purification: Purified

Immunogen: Purified His-tagged protein was used to produced this monoclonal SUMO-2 antibody.

Isotype: IgG2b

Application Details

Application Notes: Titration of the SUMO-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SUMO-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SUMO2

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Alternative Name: SUMO-2

Background: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.

UniProt: P61956

Pathways: Methionine Biosynthetic Process