CCDC99 antibody

Catalog No. ABIN4966399

This Rabbit Polyclonal antibody specifically detects CCDC99 in WB. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for CCDC99 antibody (ABIN4966399)

Target: CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCDC99 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCDC99 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arabidopsis thaliana, Helianthus annus, Hordeum vulgare, Vitis vinifera, Glycine max, Oryza sativa, Brachypodium distachyon, Zea mays, Sorghum bicolor, Solanum lycopersicum, Physcomitrella patens

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540

Application Details

Application Notes: 1 : 500 with ECL (WB)

Comment:

This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water to each tube.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for CCDC99

Target: CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))

Alternative Name: SPINDLY (SPY)

Background: SPY (SPINDLY) is O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.

Molecular Weight: 101.4 kDa

UniProt: Q96301