SQSTM1 antibody (C-Term)

Catalog No. ABIN497992

This Rabbit Polyclonal antibody specifically detects SQSTM1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SQSTM1 antibody (C-Term) (ABIN497992)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SQSTM1 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat

Purification: Immunoaffinity Chromatography

Immunogen: SQSTM1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of Human SQSTM1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: SQSTM1 antibody can be used for the detection of SQSTM1 at 1-2 μg/mL. Positive Control: Human Spleen Tissue Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt store the antibody (in aliquots) at -20 °C.

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62

Gene ID: 8878

NCBI Accession: NP_001135770

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy