SQSTM1 antibody (AA 136-400)

Catalog No. ABIN7435412

Product Details anti-SQSTM1 Antibody

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Binding Specificity: AA 136-400

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Sequestosome 1 (SQSTM1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SQSTM1. It has been selected for its ability to recognize SQSTM1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Sequestosome 1 (SQSTM1) corresdonding to Val136~Gln400 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: Sequestosome 1 (SQSTM1 Products)

Synonyms: SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody

Background: OSIL, PDB3, ZIP3, p62, p62B, p60, A170, Paget Disease Of Bone 3, Oxidative Stress Induced Like, EBI3-associated protein of 60 kDa, Ubiquitin-binding protein p62

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy