HSD17B10 antibody

Catalog No. ABIN498183

Product Details anti-HSD17B10 Antibody

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of HADH2 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for HSD17B10

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Alternative Name: HSD17B10 / ERAB (HSD17B10 Products)

Background: β-Amyloid is a neurotoxic peptide that is associated with the pathogenesis of Alzheimer's disease. β-Amyloid aggregates induce cell death of neurons through the disruption of cell membranes and the generation of reactive oxygen intermediates.These neurotoxic effects are also attributed to the interaction of β-Amyloid with intracellular proteins, specifically ERAB, the endoplasmic reticulum-associated β-Amyloid-binding protein. ERAB is characterized as a NAD+-dependent dehydrogenase that is constitutively expressed in tissues and overexpressed in neurons affected in Alzheimer's disease. Cells overexpressing ERAB in vitro have been shown to be more sensitive to β-Amyloid-induced stress, and blocking the activity of ERAB has been shown to inhibit this cell death, indicating that β-Amyloid induced cell death is mediated by ERAB.Synonyms: 17-beta-HSD10, 17-beta-hydroxysteroid dehydrogenase 10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA dehydrogenase type II, 3-hydroxyacyl-CoA dehydrogenase type-2, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, HADH2, MRPP2, Mitochondrial Marker, Mitochondrial ribonuclease P protein 2, SCHAD, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH, XH98G2

Molecular Weight: approx. 27 kDa

Gene ID: 3028

NCBI Accession: NP_001032900

UniProt: Q99714