ADCY5 antibody

Catalog No. ABIN498295

Product Details anti-ADCY5 Antibody

Target: ADCY5 (Adenylate Cyclase 5 (ADCY5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADCY5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of A Cyclase V/VI protein. (region surrounding Phe1052)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: ADCY6

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 -1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADCY5

Target: ADCY5 (Adenylate Cyclase 5 (ADCY5))

Alternative Name: Adenylate Cyclase Type 5 (ADCY5 Products)

Background: A cyclase V, also known as ADCY5, is a 1,261 amino acid Adenylyl cyclase that localizes to cellular membranes and contains two guanylate cyclase domains. Similar to other A cyclase proteins, A cyclase V uses magnesium as a cofactor to catalyze the conversion of ATP to cAMP. A cyclase VI, also known as ADCY6 (adenylate cyclase type 6), is a 1,168 amino acid A cyclase that localizes to the membrane and contains two guanylate cyclase domains. Using magnesium as a cofactor, A cyclase VI functions as a calcium-inhibitable A cyclase that catalyzes the conversion of ATP to 3',5'-cyclic AMP and diphosphate and plays a role in a variety of events throughout the body. Multiple isoforms of A cyclase VI exist due to alternative splicing events.Synonyms: ADCY5, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5

Molecular Weight: approx. 138 kDa

Gene ID: 111

NCBI Accession: NP_899200

UniProt: O95622

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma