APOE antibody

Catalog No. ABIN498561

This anti-APOE antibody is a Rabbit Polyclonal antibody detecting APOE in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for APOE antibody (ABIN498561)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOE Antibody

Specificity: This antibody detects endogenous levels of ApoE protein. (region surrounding Met86)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apolipoprotein E (Apo E)

Background: Apolipoprotein-E (apoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles. It can serve as a ligand for several lipoprotein receptors, including the LDL (apoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor. apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20 % of VLDL (very low density lipoprotein) and 1-2 % of HDL (high density lipoprotein). Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by one to two amino acids. Estrogen receptor has been shown to upregulate apoE gene expression via the ERamediated pathway, indicating a potential role for apoE in atherosclerosis. This is consistent with studies in mice in which plasma apoE levels were raised, thereby protecting the mice from diet-induced atherosclerosis. apoE has also been shown to be a potent inhibitor of proliferation and thus may play a role in angiogenesis, tumor cell growth and metastasis.Synonyms: Apo-E, ApoE

Molecular Weight: approx. 32 kDa

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism