AMPK gamma 1/2/3 (AA 280-330) antibody (AbBy Fluor® 680)

Catalog No. ABIN4996452

This Rabbit Polyclonal antibody specifically detects in WB and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AMPK gamma 1/2/3 (AA 280-330) antibody (AbBy Fluor® 680) (ABIN4996452)

Target: AMPK gamma 1/2/3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 280-330

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PRKAG2/AMPK_2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: AMPK gamma 1/2/3

Alternative Name: AMPK gamma 1/ 2/ 3

Background:

Synonyms: 5'-AMP-activated protein kinase subunit gamma-2, AAKG, AMPK gamma2, AMPK gamma 2, AMPK subunit gamma 2, CMH6, H91620p, AMPKg, AMPK subunit gamma-1, AMPK gamma1, AMPKg3, AMPK subunit gamma-3, AMPK gamma3.

Background: AMPK is a heterotrimeric complex comprising a catalytic a subunit and regulatory b and g subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKa1 and AMPKa2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKb1 encodes a 271 amino acid protein and human AMPKb2 encodes a 272 amino acid protein. The human AMPKg1 gene encodes a 331 amino acid protein. Human AMPKg2 and AMPKg3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

Gene ID: 5571, 51422, 53632