RYBP antibody

Catalog No. ABIN499732

This Rabbit Polyclonal antibody specifically detects RYBP in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RYBP antibody (ABIN499732)

Target: RYBP (RING1 and YY1 Binding Protein (RYBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RYBP Antibody

Specificity: DEDAF antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human DEDAF. The sequence is identical to that of mouse origin.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human DEDAF Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.5 to 1 μg/mL. A band at approximately 32 kDa can be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for RYBP

Target: RYBP (RING1 and YY1 Binding Protein (RYBP))

Alternative Name: RYBP

Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.Synonyms: AAP-1, APAP-1, Apoptin-associating protein 1, DED-associated factor, DEDAF, Death effector domain-associated factor, RING1 and YY1-binding protein, YEAF1, YY1 and E4TF1-associated factor 1

Gene ID: 23429

UniProt: Q8N488