LIPE antibody (C-Term)

Catalog No. ABIN500178

This anti-LIPE antibody is a Rabbit Polyclonal antibody detecting LIPE in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for LIPE antibody (C-Term) (ABIN500178)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIPE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-LIPE Antibody

Binding Specificity: C-Term

Specificity: This antibody detects LIPE.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: Lipe antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human lipe.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Alternative Name: LIPE

Background: Although initially described as an adipocyte-specific triacylglycerol lipase, lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that lipe plays a major role in adipocyte proliferation.Synonyms: EC=3.1.1.79, HSL, Hormone-sensitive lipase, LIPE

Gene ID: 3991

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism