For best experience we recommend to activate Javascript in your browser.

Histone 3 antibody (AA 1-50)

Name: Histone 3 antibody (AA 1-50)
SKU: ABIN5004684
Price: 472.31 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IHC (p) and IF (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

K562 cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 antibody (AA 1-50))

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 antibody (AA 1-50))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 antibody (AA 1-50))

IHC (p)

HeLa cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 antibody (AA 1-50))

4 images

(2 references)

Catalog No. ABIN5004684

Quick Overview for Histone 3 antibody (AA 1-50) (ABIN5004684)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

1344
908
810
41
39
39
34
33
26
23
21
21
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Human, Mouse, Rat

Host

Mouse

Clonality

Monoclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1028
418
374
323
253
202
188
187
162
159
148
127
114
58
48
48
35
22
16
14
7
7
6
4
3
1
1
1
1

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone

3G1

Discover our top product H3 Primary Antibody

Binding Specificity
52

50

48

44

39

37

37

36

35

35

34

33

32

31

31

30

23

23

23

22

21

19

18

17

17

17

16

16

16

16

15

15

14

13

13

13

13

12

12

12

11

11

10

10

9

9

9

8

8

8
AA 1-50

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein G.

Immunogen

Recombinant human HIST3H3 Protein

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IF, IHC, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Tian, Wu, Guo, Ma, Huang, Zhao: "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." in: International journal of molecular medicine, Vol. 39, Issue 4, pp. 869-878, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

Synonyms: Histone Cluster 3, H3, H3 Histone Family, Member T, Histone 3, H3, H3FT, H3/G, H3/T, H3t, H3.4 , Histone H3.1t , HIST3H3, HGNC:4778, H31T_HUMAN
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Gene ID

8290

UniProt

Q16695