Histone 3 antibody (AA 1-50)

Catalog No. ABIN5004684

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IHC (p) and IF (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for Histone 3 antibody (AA 1-50) (ABIN5004684)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 3G1

Product Details anti-Histone 3 Antibody

Binding Specificity: AA 1-50

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G.

Immunogen: Recombinant human HIST3H3 Protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN5004684)

Tian, Wu, Guo, Ma, Huang, Zhao: "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." in: International journal of molecular medicine, Vol. 39, Issue 4, pp. 869-878, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background:

Synonyms: Histone Cluster 3, H3, H3 Histone Family, Member T, Histone 3, H3, H3FT, H3/G, H3/T, H3t, H3.4 , Histone H3.1t , HIST3H3, HGNC:4778, H31T_HUMAN

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Gene ID: 8290

UniProt: Q16695