NSF antibody (AA 151-250) (AbBy Fluor® 680)

Catalog No. ABIN5007885

This Rabbit Polyclonal antibody specifically detects NSF in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for NSF antibody (AA 151-250) (AbBy Fluor® 680) (ABIN5007885)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NSF Antibody (AbBy Fluor® 680)

Binding Specificity: AA 151-250

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NSF

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background:

Synonyms: N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor like protein, N ethylmaleimide sensitive fusion protein, N ethylmaleimide sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, NEM sensitive fusion protein, NEM sensitive fusion protein, NEM-sensitive fusion protein, NSF, NSF_HUMAN, SKD 2, SKD2, SKD2, Vesicle fusing ATPase, Vesicle fusing ATPase, Vesicle-fusing ATPase, Vesicular fusion protein NSF, Vesicular fusion protein NSF, Vesicular-fusion protein NSF.

Background: Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the -SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the -SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).