SKA1 antibody (AA 181-255) (AbBy Fluor® 750)

Catalog No. ABIN5010577

The Rabbit Polyclonal anti-SKA1 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect SKA1 in samples from Human.

Quick Overview for SKA1 antibody (AA 181-255) (AbBy Fluor® 750) (ABIN5010577)

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA1 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SKA1 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 181-255

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SKA1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SKA1

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Alternative Name: SKA1

Background:

Synonyms: Ska1, SKA1_HUMAN, Spindle and kinetochore associated complex subunit 1, Spindle and kinetochore associated protein 1, Spindle and kinetochore-associated protein 1, Spindle and KT kinetochore associated 1, Spindle and KT associated 1, C18orf24, MGC10200.

Background: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.

Pathways: M Phase