TWIST1, TWIST2 (AA 75-160) antibody (AbBy Fluor® 750)

Catalog No. ABIN5012109

This Rabbit Polyclonal antibody specifically detects in IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for TWIST1, TWIST2 (AA 75-160) antibody (AbBy Fluor® 750) (ABIN5012109)

Target: TWIST1, TWIST2

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: AbBy Fluor® 750

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 75-160

Specificity: This antibody will detect TWIST1 and TWIST2

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TWIST2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TWIST1, TWIST2

Alternative Name: TWIST1 and TWIST2

Background:

Synonyms: TWIST, bHLHa38, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, TWIST1, bHLHa39, Class A basic helix-loop-helix protein 39, Dermis expressed protein 1, Dermis-expressed protein 1, DERMO 1, Dermo-1, DERMO1, MGC117334, Twist 2, Twist homolog 2 Drosophila, Twist homolog 2, Twist related bHLH protein Dermo1, Twist related protein 2, Twist-related protein 2, Twist2, TWST2_HUMAN.

Background: TWIST1 and TWIST2 are basic helix-loop-helix transcriptional repressors that bind to E boxes in gene promoters, acting as master regulators in a variety of biological processes, including organogenesis, osteogenesis, cancer progression and hematopoietic cell development. Both TWIST1 and TWIST2 are found to act as Homodimers or Heterodimers with another bHLH protein. TWIST1 Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses expression of proinflammatory cytokines such as TNFA and IL1B. TWIST2 inhibits transcriptional activation by MYOD1, MYOG, MEF2A, and MEF2C. Twist2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis.

Gene ID: 7291, 117581

UniProt: Q15672, Q8WVJ9