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alpha Adducin antibody (AA 99-328)

This Rabbit Polyclonal antibody specifically detects alpha Adducin in WB, IHC, IP and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN5013084

Quick Overview for alpha Adducin antibody (AA 99-328) (ABIN5013084)

Target

See all alpha Adducin (ADD1) Antibodies
alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity

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  • 6
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  • 4
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Human

Host

  • 90
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Rabbit

Clonality

  • 89
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Polyclonal

Conjugate

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This alpha Adducin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    AA 99-328

    Purpose

    Polyclonal Antibody to Alpha Adducin (ADD1)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against ADD1. It has been selected for its ability to recognize ADD1 in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Mouse, Pig, Rat

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    ADD1 (Thr99-Gln328)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

    Preservative

    Dithiothreitol (DTT), ProClin

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

    Alternative Name

    ADD1

    Background

    ADDA, Adducin 1, Erythrocyte adducin subunit alpha

    Pathways

    Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling
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