NCK1 antibody (AA 2-377)

Catalog No. ABIN5014021

This anti-NCK1 antibody is a Rabbit Polyclonal antibody detecting NCK1 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for NCK1 antibody (AA 2-377) (ABIN5014021)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NCK1 Antibody

Binding Specificity: AA 2-377

Purpose: Polyclonal Antibody to Cytoplasmic Protein NCK1 (NCK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against NCK1. It has been selected for its ability to recognize NCK1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPC712Hu01Recombinant Cytoplasmic Protein NCK1 (NCK1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background: NCKalpha, NCKa, NCK adaptor protein 1, Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1, SH2/SH3 adaptor protein NCK-alpha

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals