Ubiquitin antibody

Catalog No. ABIN5024488

This Mouse Monoclonal antibody specifically detects Ubiquitin in WB, IHC, ELISA, ICC, IF and IP. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Ubiquitin antibody (ABIN5024488)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: FK2

Product Details anti-Ubiquitin Antibody

Specificity: Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.

Purification: Protein A Purified

Immunogen: Ubiquitin conjugated lysozyme

Isotype: IgG1

Application Details

Application Notes:

• WB (1:10000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:5000 dilution of ABIN5024488 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Ubiquitin antibody (ABIN5024488)

Nombela, Requena-Platek, Morales-Lange, Chico, Puente-Marin, Ciordia, Mena, Coll, Perez, Mercado, Ortega-Villaizan: "Rainbow Trout Red Blood Cells Exposed to Viral Hemorrhagic Septicemia Virus Up-Regulate Antigen-Processing Mechanisms and MHC I&II, CD86, and CD83 Antigen-presenting Cell Markers." in: Cells, Vol. 8, Issue 5, (2019) (PubMed).

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway