Ubiquitin antibody (Biotin)

Catalog No. ABIN5024521

This anti-Ubiquitin antibody is a Mouse Monoclonal antibody detecting Ubiquitin in WB, IHC, ELISA, ICC, IF and IP. Suitable for Human.

Quick Overview for Ubiquitin antibody (Biotin) (ABIN5024521)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: FK2

Product Details anti-Ubiquitin Antibody (Biotin)

Specificity: Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.

Purification: Protein A Purified

Immunogen: Ubiquitin conjugated lysozyme

Isotype: IgG1

Application Details

Application Notes:

• WB (1:10000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:5000 dilution of ABIN5024521 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway