CD3 epsilon antibody (AA 2-203)

Catalog No. ABIN5027093

This anti-CD3 epsilon antibody is a Mouse Monoclonal antibody detecting CD3 epsilon in WB and IHC. Suitable for Human.

Quick Overview for CD3 epsilon antibody (AA 2-203) (ABIN5027093)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ABM45B3

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 2-203

Purification: Protein G Chromatography

Immunogen: A partial length recombinant CD3e protein (amino acids 2-203) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 0.5-1 μg/mL, IHC: 5-10 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: CD3e (CD3-epsilon), together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The gene for CD3e have been mapped to band q23 of chromosome 11 in humans and consisted of nine exons. Three exons, encoding the junction of leader peptide and mature protein, are extremely small. The CD3e cytoplasmic tail, highly conserved, can be tentatively subdivided into three regions, the N-terminal region contains a basic amino acid cluster, the central region contains a proline-rich sequence, and the C-terminal region contains the ITAM . Among the CD3 subunits, CD3e is the most likely candidate for performing a critical role in the TCR signaling response. CD3e is represented twice in the TCR complex, serving as a component of both the gamma/epsilon and delta/epsilon dimers. In addition, after TCR cross-linking, CD3e and zeta are the predominant tyrosine-phosphorylated TCR subunits.

Molecular Weight: 22 kDa

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway