ATG4B antibody (AA 94-106) (Atto 390)

Catalog No. ABIN5066012

This anti-ATG4B antibody is a Rabbit Polyclonal antibody detecting ATG4B in WB, IF and ICC. Suitable for Human.

Quick Overview for ATG4B antibody (AA 94-106) (Atto 390) (ABIN5066012)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATG4B Antibody (Atto 390)

Binding Specificity: AA 94-106

Specificity: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.,Detects ~44 kDa.

Cross-Reactivity: Human, Mouse

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066012 was sufficient for detection of ATG4B in 15 μg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background: Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation.

Gene ID: 23192

NCBI Accession: NP_037457

UniProt: Q9Y4P1

Pathways: Autophagy