FNIP1 antibody

Catalog No. ABIN5066839

The Rabbit Polyclonal anti-FNIP1 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect FNIP1 in samples from Human. There is 1 publication available.

Quick Overview for FNIP1 antibody (ABIN5066839)

Target: FNIP1 (Folliculin Interacting Protein 1 (FNIP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FNIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-FNIP1 Antibody

Specificity: Strong expression is found in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels detected in clear cell renal cell carcinoma (RCC) and chromophobe RCC than in normal kidney tissue.,Detects ~130 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the mid-protein of Human FNIP1

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066839 was sufficient for detection of FNIP1 in 15 μg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-FNIP1 antibody (ABIN5066839)

Zhang, Chen, Yu, Cui, Chen, Yi, Wang, Wei, Liang, Ye, Zhou: "FNIP1 suppresses colorectal cancer progression through inhibiting STAT3 phosphorylation and nuclear translocation." in: iScience, Vol. 27, Issue 9, pp. 110730, (2024) (PubMed).

Target Details for FNIP1

Target: FNIP1 (Folliculin Interacting Protein 1 (FNIP1))

Alternative Name: FNIP1

Background: Folliculin-interacting protein 1 (FNIP1) is a co-chaperone of HSP90 (1). It Inhibits the ATPase activity of HSP90AA1 leading to reduction in its chaperone activity. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways and may regulate the phosphorylation of RPS6KB1 (2). FNIP1 is strongly expressed in the heart, liver placenta, muscle, nasal mucosa, salivary gland, and uvula. Elevated levels of FNIP1 have been detected in renal cell carcinoma (RCC) (3).

Gene ID: 96459

NCBI Accession: NP_001008738

UniProt: Q8TF40