Arginylation (N-Term) antibody (FITC)

Catalog No. ABIN5066977

The Mouse Monoclonal anti- antibody has been validated for WB and FACS. It is suitable to detect in samples from .

Quick Overview for Arginylation (N-Term) antibody (FITC) (ABIN5066977)

Target: Arginylation

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4A9

Product Details

Binding Specificity: N-Term

Specificity: Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Purification: Protein G Purified

Immunogen: Synthetic N-terminal arginylated peptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• FCM (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066977 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Arginylation

Target Type: Chemical

Background: Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).