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Dityrosine antibody (HRP)

This anti-Dityrosine antibody is a Mouse Monoclonal antibody detecting Dityrosine in WB, ELISA, ICC and IF. Suitable for .
Catalog No. ABIN5067464

Quick Overview for Dityrosine antibody (HRP) (ABIN5067464)

Target

Dityrosine (DT)

Host

  • 12
Mouse

Clonality

  • 12
Monoclonal

Conjugate

  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Dityrosine antibody is conjugated to HRP

Application

  • 12
  • 11
  • 10
  • 10
  • 10
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone

7D4
  • Specificity

    Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

    Purification

    Protein G Purified

    Immunogen

    Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

    Isotype

    IgG1
  • Application Notes

    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.

    Comment

    A 1:1000 dilution of ABIN5067464 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Conjugated antibodies should be stored at 4°C
  • Target

    Dityrosine (DT)

    Alternative Name

    Dityrosine

    Target Type

    Dipeptide

    Background

    ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
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