Dityrosine antibody (HRP)
Quick Overview for Dityrosine antibody (HRP) (ABIN5067464)
Target
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
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Purification
- Protein G Purified
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Immunogen
- Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
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Isotype
- IgG1
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Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
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Comment
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A 1:1000 dilution of ABIN5067464 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Dityrosine (DT)
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Alternative Name
- Dityrosine
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Target Type
- Dipeptide
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Background
- ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
Target
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