PEX14 antibody (N-Term, pHis6)

Catalog No. ABIN5326727

This Rabbit Polyclonal antibody specifically detects PEX14 in WB. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for PEX14 antibody (N-Term, pHis6) (ABIN5326727)

Target: PEX14 (Peroxisomal Biogenesis Factor 14 (PEX14))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEX14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PEX14 Antibody

Binding Specificity: N-Term, pHis6

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Arabidopsis thaliana, Magnaporthe oryzae

Characteristics: Expected / apparent Molecular Weight of the Antigene: 55.5 / 75-65 kDa (depending upon the gel system)

Purification: serum

Immunogen: Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p (Q9FE40, At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 were replaced with a dual StrepII-His6 tag.

Application Details

Application Notes: 1: 10 000 with standard ECL (WB)

Comment:

Antibodies will detect Pex14 protein in both light- and dark-grown seedlings grown on petri dishes and in rosette leaves of adult plants grown in soil.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for PEX14

Target: PEX14 (Peroxisomal Biogenesis Factor 14 (PEX14))

Alternative Name: Pex14p

Background: AGI Code: At5g62810
Pex14p has a protein transporter activity and is involved in protein targeting into peroxisome. Submitted protein name: Genomic DNA, chromosome 5, P1 clone:MQB2

Molecular Weight: expected: 55.5 kDa, apparent: 75-65 kDa (depending upon the gel system)

UniProt: Q9FE40

Pathways: Maintenance of Protein Location