SUPT5H antibody (N-Term)

Catalog No. ABIN5514714

This Rabbit Polyclonal antibody specifically detects SUPT5H in WB. It exhibits reactivity toward Human.

Quick Overview for SUPT5H antibody (N-Term) (ABIN5514714)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SUPT5H Antibody

Binding Specificity: N-Term

Sequence: SAAGSEKEEE PEDEEEEEEE EEYDEEEEEE DDDRPPKKPR HGGFILDEAD

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SUPT5H

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: SUPT5H

Background: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Alias Symbols: SPT5, SPT5H, Tat-CT1

Protein Size: 1087

Gene ID: 6829

NCBI Accession: NM_001111020, NP_001104490

UniProt: O00267