GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 351-367) Primary Antibody
GJA1
Reactivity: Human, Rat
ICC, IHC (fro), IHC (p), WB
Host: Rabbit
Polyclonal
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Catalog No. ABIN5518645
$280.00
Plus shipping costs $45.00
100 μg
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- Target
- Binding Specificity
- AA 351-367, C-Term
- Reactivity
- Human, Rat
- Host
- Rabbit
- Clonality
- Polyclonal
- Conjugate
- This GJA1 antibody is un-conjugated
- Application
- Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- Sequence
- HELQPLAIVD QRPSSRA
- Cross-Reactivity (Details)
- Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Characteristics
- Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: gap junction protein, alpha 1, 43 kDa
Protein Name: Gap junction alpha-1 protein - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(351-367aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences.
- Isotype
- IgG
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- Application Notes
- WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Sodium azide, Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Xu, Hu, Khan, Shi, Cong, Li, Dai, Dai: "Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43." in: Acta pharmacologica Sinica, Vol. 37, Issue 2, pp. 246-54, 2017 (PubMed).
Zeng, Li, Li, Li, Xiao, Chu, Yang: "Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats." in: Bioengineered, Vol. 7, Issue 5, pp. 314-320, 2017 (PubMed).
Ming, Zhou, Du, Fan, Pan, Wang, Fan, Jiang: "Identification of miR-200a as a novel suppressor of connexin 43 in breast cancer cells." in: Bioscience reports, Vol. 35, Issue 5, 2016 (PubMed).
Ming, Zhou, Du, Fan, Pan, Wang, Fan, Jiang: "miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells." in: Bioscience reports, Vol. 35, Issue 6, 2016 (PubMed).
Zhang, Gao, Dong, Wang, Yao, Zhang, Hu, Xu, Zuo, Wang, Zhou, Zhao, Peng: "The compound Chinese medicine "Kang Fu Ling" protects against high power microwave-induced myocardial injury." in: PLoS ONE, Vol. 9, Issue 7, pp. e101532, 2014 (PubMed).
Li, Tang, Liang, Li, Wang, Song, Zheng, Xi, Zhang, Hescheler, Zhu: "Coculture of embryonic ventricular myocytes and mouse embryonic stem cell enhance intercellular signaling by upregulation of connexin43." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 1, pp. 53-63, 2013 (PubMed).
Xing, Lv, Wang, Yan, Zhao, Cao: "Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats." in: Journal of biomedical science, Vol. 19, pp. 6, 2012 (PubMed).
Zhang, Yu, Dai, Cheng, Zhang, Dai: "CPU86017-RS attenuate hypoxia-induced testicular dysfunction in mice by normalizing androgen biosynthesis genes and pro-inflammatory cytokines." in: Acta pharmacologica Sinica, Vol. 33, Issue 4, pp. 470-8, 2012 (PubMed).
Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, 2010 (PubMed).
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Xu, Hu, Khan, Shi, Cong, Li, Dai, Dai: "Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43." in: Acta pharmacologica Sinica, Vol. 37, Issue 2, pp. 246-54, 2017 (PubMed).
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- Target
- Alternative Name
- GJA1 (GJA1 Antibody Abstract)
- Background
- Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Synonyms: Connexin 43 antibody|Connexin-43 antibody|Connexin43 antibody|Cx 43 antibody|CX43 antibody|CXA1_HUMAN antibody|DFNB38 antibody|Gap junction 43 kDa heart protein antibody|Gap junction alpha 1 protein antibody|Gap junction alpha-1 protein antibody|Gap junction protein alpha 1 43kD antibody|Gap junction protein alpha 1 43 kDa(connexin 43) antibody|Gap junction protein alpha 1 43 kDa antibody|Gap junction protein alpha like antibody|GJA 1 antibody|Gja1 antibody|GJAL antibody|HGNC:4282 antibody|HGNC:8112 antibody|Oculodentodigital dysplasia antibody|ODD antibody|ODDD antibody|ODOD antibody|SDTY 3 antibody|SDTY3 antibody|Syndactyly type III antibody - UniProt
- P17302
- Pathways
- MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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